A Triple-Arginine Motif in the Amino-Terminal Domain and Oligomerization Are Required for HIV-1 Inhibition by Human MX2

Autor:	Goujon, Caroline, Greenbury, Rebecca A., Papaioannou, Stelios, Doyle, Tomas, Malim, Michael H.
Jazyk:	angličtina
Rok vydání:	2015
Předmět:	Myxovirus Resistance Proteins Amino Acid Motifs Immunoblotting Molecular Sequence Data HIV-1 Humans Arginine Flow Cytometry Fluorescent Antibody Technique Indirect Virus-Cell Interactions Polymerization
Zdroj:	Journal of Virology
ISSN:	1098-5514 0022-538X
Popis:	We have employed molecular genetic approaches to understand the domain organization of the HIV-1 resistance factor myxovirus resistance 2 (MX2). First, we describe an essential triple-arginine motif in the amino-terminal domain. Second, we demonstrate that this 91-residue domain mediates antiviral activity when appended to heterologous proteins, and we provide genetic evidence that protein oligomerization is required for MX2 function. These insights will facilitate future work aiming to elucidate MX2's mechanism of action.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::a9fa6cfbb1591a97bc57897e4838eb00 http://europepmc.org/articles/PMC4442396 Zobrazit plný text záznamu