Identification of Novel Mycobacterial Targets for Murine CD4

Autor: Alison J, Johnson, Steven C, Kennedy, Tony W, Ng, Steven A, Porcelli
Rok vydání: 2018
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 1808
ISSN: 1940-6029
Popis: Enzyme-linked immunospot (ELISPOT) is an assay used to detect secretion of cytokines from immune cells. The resolution and sensitivity of ELISPOT allow for the detection of rare T cell specificities and small quantities of molecules produced by individual cells. In this chapter, we describe an epitope screening method that uses CD4
Databáze: OpenAIRE