Cadmium-induced apoptosis of primary epithelial lung cells: involvement of Bax and p53, but not of oxidative stress
| Autor: | M, Lag, S, Westly, T, Lerstad, C, Bjørnsrud, M, Refsnes, P E, Schwarze |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Dose-Response Relationship Drug Apoptosis Epithelial Cells Acetates Nitric Oxide Rats Inbred WKY Rats Rats Sprague-Dawley Oxidative Stress Proto-Oncogene Proteins c-bcl-2 Proto-Oncogene Proteins Animals Tumor Suppressor Protein p53 Reactive Oxygen Species Lung Cells Cultured Cadmium bcl-2-Associated X Protein |
| Zdroj: | Cell biology and toxicology. 18(1) |
| ISSN: | 0742-2091 |
| Popis: | The lung is a target organ for cadmium (Cd) toxicity. Apoptosis induced by cadmium acetate (CdAc) was studied in alveolar type 2 cells and Clara cells isolated from rat lung. Relatively low concentrations of CdAc (1-10 micromol/L) induced apoptosis after exposure for 20 h. Type 2 cells were more sensitive than Clara cells to Cd-induced apoptosis and loss of cell viability. On exposure to 10 micromol/L CdAc, the levels of the apoptosis-modulating proteins p53 and Bax were increased at 2 h and 5-12 h, respectively. The expression of p53 preceded the expression of Bax and the apoptotic process. The exposure to 10 micromol/L CdAc did not significantly increase the formation of cellular reactive oxygen species (ROS). However, after exposure to a high concentration of CdAc (100 micromol/L), a 30% increase of the ROS level was observed. No significant nitric oxide production was measured following CdAc exposure. Catalase, superoxide dismutase, dimethyl sulfoxide, or tetramethylthiourea did not protect against Cd-induced apoptosis. In conclusion, the results show that Clara cells and type 2 cells are sensitive to Cd-induced apoptosis. Increased levels of p53 and Bax are suggested to be involved in the apoptosis. The apoptosis did not appear to be mediated by oxidative pathways. |
| Databáze: | OpenAIRE |
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