[Engineering by reverse genetics and characterization of the new reassortant influenza virus strain H5N1]
| Autor: | A D, Zeberezhnyĭ, T V, Grebennikova, G K, Vorkunova, A G, Yuzhakov, L V, Kostina, S N, Norkina, T I, Aliper, E A, Nepoklonov, D K, Lvov |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Influenza A Virus
H5N1 Subtype Hemagglutinin Glycoproteins Influenza Virus Chick Embryo Antibodies Viral Virus Replication Reverse Genetics Influenza A Virus H1N1 Subtype Vaccines Inactivated Influenza Vaccines Influenza in Birds Animals Humans Genetic Engineering Antigens Viral Chickens Reassortant Viruses |
| Zdroj: | Voprosy virusologii. 59(6) |
| ISSN: | 0507-4088 |
| Popis: | Reverse genetics was applied to engineering of the reassortantvaccine candidate strain against highly pathogenic avian influenza viruses (HPAIVs) of the H5 subtype. The new strain recPR8-H5N1 contains the HA gene from the Russian HPAIV A/Kurgan/05/2005 (H5N1), the NA and internal genes from A/Puerto Rico/8/34 (H1N1). The strain recPR8-H5N1 demonstrated the antigenic specificity (H5), high proliferation rate in 12 days chicken embryos, and was lethal for the embryos in 36 hours. An inactivated emulsified vaccine based on the strain recPR8-H5N1 elicited high antibody titers and protected 6-week-old chickens from lethal challenge with the HPAIV A/Kurgan/05/2005 (H5N1) on day 21 after single immunization. Infection of non-vaccinated birds with the strain recPR8-H5N1 did not cause any pathology, and the virus was not detected using PCR in blood and cloacal swabs on day 7 p.i. Specific weak seroconversion caused by infection with the strain recPR8-H5N1 was detected on day 14 p.i. As a result, a new influenza virus strain was obtained with modified properties. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|