| Autor: |
V E, Illarionova, T M, Taratina, L A, Volkova, S F, Bartoshevich, B V, Moskvichev |
| Rok vydání: |
1989 |
| Předmět: |
|
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia. 25(1) |
| ISSN: |
0555-1099 |
| Popis: |
The gyration radius (R0) of native streptokinase (SK) was found to be R0 = (40 +/- ) A by small-angle X-ray scattering. Experimental hydrodynamic characteristics of SK were S0(20),W = (2.8 +/- 0.1)S; D0(20),W = (6.0 +/- 0.5) x 10(-7) cm2/s; [n] = 0.12 dl/g. The molecular weight of the enzyme was found to be 44,000. The values of the form factor R0/Rsphere = 2.1 and the frictional ratio f/f0 = 1.5 indicate considerable anisometry of the SK molecule. Basing on the curves of small-angle X-ray scattering of SK modified with a synthetic linear copolymer of N-vinylpyrrolidone (P) at a molar ratio SK less than P, a structural model of the conjugate was proposed. The modified form consisted of a dense nucleus covered with a diffuse polymeric membrane. In accordance with the model, R0 of modified SK and of the whole conjugate were found to be R0nucleus = (34 +/- 2) A and R0conjugate = (114 +/- 5)A. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
