Correlation of the in situ detection of polymerase chain reaction-amplified metalloproteinase complementary DNAs and their inhibitors with prognosis in cervical carcinoma
| Autor: | G J, Nuovo, P B, MacConnell, A, Simsir, F, Valea, D L, French |
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| Rok vydání: | 1995 |
| Předmět: |
Tissue Inhibitor of Metalloproteinase-2
DNA Complementary Base Sequence Molecular Sequence Data Metalloendopeptidases Proteins Uterine Cervical Neoplasms Tissue Inhibitor of Metalloproteinases Prognosis Polymerase Chain Reaction Matrix Metalloproteinase 9 Gelatinases Humans Matrix Metalloproteinase 2 Female Collagenases RNA Messenger RNA Neoplasm Glycoproteins |
| Zdroj: | Cancer research. 55(2) |
| ISSN: | 0008-5472 |
| Popis: | The purpose of this study was to correlate the presence of matrix metalloproteinase (MMP)-9 and MMP-2 and tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2 mRNAs, detected in serial sections using the reverse transcriptase in situ PCR technique, with prognosis in 23 cases of cervical carcinoma. PCR-amplified MMP and TIMP cDNA were restricted to the invasive cancers cells and the surrounding stromal cells. The ratios of cancer and stromal cells expressing MMP-9 and MMP-2 to those expressing TIMP-1 and TIMP-2 were approximately 1 in those cancers with a good prognosis. This MMP:TIMP ratio in the cancer and stromal cells with a poor prognosis was significantly increased to 5.4 and 3.4 (P0.0001), respectively, reflecting a marked reduction in the TIMP detection rate in cancers with a poor prognosis. In cervical cancer cell lines SiHa and HeLa, the MMP:TIMP ratio was also close to 1 and, interestingly, these cell lines are invasive but rarely metastatic in nude mice. These data suggest that the balance of MMP-9 and MMP-2 to TIMP-1 and TIMP-2 expression is an essential factor in the aggressiveness of cervical cancer. |
| Databáze: | OpenAIRE |
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