Oily fraction of Semecarpus anacardium Linn nuts involves protein kinase C activation for its pro-inflammatory response
| Autor: | Yamini B, Tripathi, Nidhi, Pandey, Deepshikha, Tripathi, Pratibha, Tripathi |
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| Rok vydání: | 2011 |
| Předmět: |
Plant Extracts
Nitric Oxide Synthase Type II Antioxidants Rats Enzyme Activation Protein Kinase C-delta Semecarpus 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Carcinogens Macrophages Peritoneal Animals Nuts Tetradecanoylphorbol Acetate Quercetin Enzyme Inhibitors Cells Cultured Signal Transduction |
| Zdroj: | Indian journal of experimental biology. 48(12) |
| ISSN: | 0019-5189 |
| Popis: | The oily fraction (non polar fraction-NPF) of S. anacardium (SA) significantly increased the expression of protein kinase C-delta (PKC-delta) in macrophages in concentration dependent manner, which was similar to phorbol myristate acetate (PMA) response. Further, H-7 (1-(5-isoquinolinesulphonyl)-2-methylpiperazine), an inhibitor of PKC significantly inhibited this NPF mediated response in a concentration dependent manner. In the post treatment kinetics, H-7 showed this inhibition only up to 6 min post NPF/PMA addition, but in similar condition, quercetin, a flavone with reported antioxidant property, showed this inhibition only up to 2 min. The results clearly suggest that oily fraction of SA nuts enhances the expression of PKC protein, which may be responsible for its reported pro-inflammatory property. |
| Databáze: | OpenAIRE |
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