Isolation of bovine mammary lymphocytes for fluorescent activated flow cytometry

Autor:	T L, Ebling, L K, Fox, K M, Byrne
Rok vydání:	2001
Předmět:	Staining and Labeling T-Lymphocytes Centrifugation Density Gradient Animals Antibodies Monoclonal Cattle Female Breast Cell Separation Flow Cytometry
Zdroj:	Methods in cell science : an official journal of the Society for In Vitro Biology. 22(2-3)
ISSN:	1381-5741
Popis:	A detailed methodology is described for the isolation of T lymphocytes from bovine mammary gland secretions for flow cytometry. Mammary gland secretions are collected and centrifuged to separate the leukocytes from the mammary supernatant. The leukocytes are purified using a density gradient and diluted for fluorescent staining. Using tri-color fluorescent staining, the T lymphocytes are identified by monoclonal antibodies and stained with secondary antibodies. Flow cytometry is used to quantify the cellular subpopulations of the T lymphocytes. Proportional and statistical analysis of the flow cytometric data is conducted with computer software, CELLQuest and SAS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::a7e6c3e4215972885291476c8df00d65 https://pubmed.ncbi.nlm.nih.gov/11264959 Zobrazit plný text záznamu Full text from SpringerLink