| Autor: |
Jelena, Plačkić, Jens, Kockskämper |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1816 |
| ISSN: |
1940-6029 |
| Popis: |
High quality cardiomyocyte isolation is of critical importance for successful studies of myocardial function at the cellular and molecular level. Although previous work has established isolation procedures for various species, it still remains challenging to produce consistently a high yield of viable and healthy cardiomyocytes. The basis for the most successful and reproducible isolation of cardiomyocytes from intact hearts is the Langendorff retrograde perfusion technique. Here, we will illustrate in detail all practical aspects of the enzyme-based Langendorff isolation of rat atrial and ventricular cardiomyocytes. This includes a series of obligatory steps starting from quick aortic cannulation to rinse the heart from blood, short perfusion of the heart with Ca |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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