Drop-off during ribosome hopping
| Autor: | A J, Herr, N M, Wills, C C, Nelson, R F, Gesteland, J F, Atkins |
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| Rok vydání: | 2001 |
| Předmět: |
Gene Expression Regulation
Viral Base Sequence Genes Viral Recombinant Fusion Proteins Molecular Sequence Data RNA Transfer Gly Maltose-Binding Proteins Open Reading Frames RNA Bacterial Thioredoxins Bacterial Proteins Protein Biosynthesis Mutation Bacteriophage T4 Nucleic Acid Conformation Amino Acid Sequence Carrier Proteins Codon Ribosomes Protein Binding |
| Zdroj: | Journal of molecular biology. 311(3) |
| ISSN: | 0022-2836 |
| Popis: | Ribosomes bypass a 50 nucleotide non-coding segment of mRNA between the two open reading frames of bacteriophage T4 gene 60 in order to synthesize a topoisomerase subunit. While nearly all ribosomes appear to initiate bypassing, only 50 % resume translation in the second open reading frame. Failure to bypass is shown here to be independent of the stop codon at the end of the first open reading frame and to be amplified by mutant variants of tRNA(Gly)(2) known to diminish bypassing efficiency. Unproductive bypassing may result from premature dissociation of peptidyl-tRNAs from ribosomes (drop-off) or resumption of translation at inappropriate sites. Assessment of the influence of factors known to induce drop-off reveals that ribosome recycling factor accounts for a small fraction of unproductive bypassing products, but none of the other known factors appear to play a significant role. Resumption of translation at inappropriate sites appears to be minimal, which suggests that spontaneous release of the peptidyl-tRNA may account for the remaining unproductive bypassing products and may be inherent to the gene 60 bypassing mechanism. |
| Databáze: | OpenAIRE |
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