| Autor: |
Fei, Gao, Jing, Yi, Guiying, Shi, Hui, Li, Xuegeng, Shi, Zhiwei, Wang, Xueming, Tang |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi. 23(1) |
| ISSN: |
0253-2727 |
| Popis: |
To investigate whether ascorbic acid could enhance the efficacy of arsenic trioxide (As(2)O(3)) combined with 2, 3-dimethoxy-1, 4-naphthoquinone (DMNQ) in inducing the apoptosis of leukemia cell line U937 and its possible mechanism.Flow cytometry and electron microscopy were applied to detect apoptosis of U937 cells after treatment with various combinations of As(2)O(3), DMNQ and ascorbic acid for 24 hours.As(2)O(3) and DMNQ induced-apoptosis of U937 cells was enhanced (35.24%--61.20%) upon cotreatment with ascorbic acid. Catalase could reverse this effect of DMNQ. Ascorbic acid had no effect on DMNQ-induced apoptosis of U937 cells.Ascorbic acid enhanced the apoptosis of U937 cells via reactive oxygen species-dependent pathway in the presence of As(2)O(3). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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