| Autor: |
M I, El-Enbaawy, Y A, Abdalla, A Z, Hussein, Roukaya M, Osman, S A, Selim |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
The Egyptian journal of immunology. 10(1) |
| ISSN: |
1110-4902 |
| Popis: |
A monoclonal antibody (mAb) against C. perfringens type D epsilon toxin was produced in hybridoma tissue culture supernatant and in BALB/C mice ascitic fluid. The mAb reacted with single band of the purified epsilon toxin and efficiently neutralized the lethal effect of epsilon toxin in mice. A competitive ELISA (C-ELISA) was developed using this mAb and used to evaluate C. perfringens type D vaccines. Individual serum samples from vaccinated rabbits were tested using C-ELISA and mouse neutralization test (MNT). The mAb based C-ELISA correlated with the conventional MNT for estimation of antitoxin level. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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