| Popis: |
The appearance of 19S and 7S antibody-forming cells (producers) in mice following a single i.p. injection of sheep erythrocytes, and their recirculation between spleen, thymus and marrow, were studied by the direct and indirect Jerne plaque techniques. The appearance and distribution of antigen-sensitive precursor cells (memory cells) in the same tissues were determined by transferring aliquots of thymus, marrow and spleen cells to irradiated recipients, restimulating with antigen, and counting the plaque-forming cells (PFCs) in the recipient spleens. 2-ME sensitive and 2-ME resistant serum antibodies in donors and recipients were also titrated. |
