Monoclonal anti-GFAP antibodies: extraction, characteristics, and immunoenzyme assay

Autor:	V P, Chekhonin, O I, Gurina, T B, Dmitrieva, I I, Shepeleva, I A, Ryabukhin, G N, Babich
Rok vydání:	2001
Předmět:	Immunoenzyme Techniques Molecular Weight Mice Mice Inbred BALB C Hybridomas Blotting Western Glial Fibrillary Acidic Protein Animals Antibodies Monoclonal Humans Reproducibility of Results Sensitivity and Specificity
Zdroj:	Bulletin of experimental biology and medicine. 132(2)
ISSN:	0007-4888
Popis:	Methods of GFAP purification and obtaining of hybridoma cells producing monoclonal anti-GFAP antibodies and properties of GFAP preparation were described. The immunobloting data on specificity of obtained monoclonal antibodies are presented. A new method of GFAP immunoenzyme assay based on GFAP preparation and anti-GFAP antibodies was elaborated. Standardization of the immunoenzyme system was shown in tests for specificity, accuracy, and reproducibility.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::a73e24f98df546031d4b3594458441a4 https://pubmed.ncbi.nlm.nih.gov/11713563 Zobrazit plný text záznamu Full text from SpringerLink