Engineering, expression in transgenic plants and characterisation of E559, a rabies virus-neutralising monoclonal antibody
| Autor: | Craig J, van Dolleweerd, Audrey Y-H, Teh, Ashley C, Banyard, Leonard, Both, Hester C T, Lotter-Stark, Tsepo, Tsekoa, Baby, Phahladira, Wonderful, Shumba, Ereck, Chakauya, Claude T, Sabeta, Clemens, Gruber, Anthony R, Fooks, Rachel K, Chikwamba, Julian K-C, Ma |
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| Rok vydání: | 2014 |
| Předmět: |
Mesocricetus
RIG Rabies Nicotiana tabacum viruses fungi food and beverages Antibodies Monoclonal Antibodies Viral Plants Genetically Modified Recombinant Proteins Disease Models Animal Mice Major Articles and Brief Reports Rabies virus monoclonal antibody Cricetinae Tobacco Viruses post-exposure prophylaxis Animals Humans |
| Zdroj: | The Journal of Infectious Diseases |
| ISSN: | 1537-6613 |
| Popis: | Rabies post-exposure prophylaxis (PEP) currently comprises administration of rabies vaccine together with rabies immunoglobulin (RIG) of either equine or human origin. In the developing world, RIG preparations are expensive, often in short supply, and of variable efficacy. Therefore, we are seeking to develop a monoclonal antibody cocktail to replace RIG. Here, we describe the cloning, engineering and production in plants of a candidate monoclonal antibody (E559) for inclusion in such a cocktail. The murine constant domains of E559 were replaced with human IgG1κ constant domains and the resulting chimeric mouse-human genes were cloned into plant expression vectors for stable nuclear transformation of Nicotiana tabacum. The plant-expressed, chimeric antibody was purified and biochemically characterized, was demonstrated to neutralize rabies virus in a fluorescent antibody virus neutralization assay, and conferred protection in a hamster challenge model. |
| Databáze: | OpenAIRE |
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