Development of a method for the identification of receptor activator of nuclear factor-κB

Autor:	Mana, Kawakami, Hisataka, Yasuda, Daisuke, Nishida, Akira, Katakura, Toshihide, Mizoguchi
Rok vydání:	2021
Předmět:	Mice Membrane Glycoproteins Receptor Activator of Nuclear Factor-kappa B NF-kappa B Animals Osteoclasts Bone Marrow Cells Carrier Proteins
Zdroj:	Journal of oral biosciences. 63(1)
ISSN:	1880-3865
Popis:	Osteoclasts are induced by macrophage colony-stimulating factor-1 (CSF-1) and receptor activator of nuclear factor-κB (RANK) ligand (RANKL). Monocyte/macrophage lineages are thought to be osteoclast precursors; however, such cells have not been fully characterized owing to a lack of tools for their identification. Osteoclast precursors express colony-stimulating factor-1 receptor (CSF-1R) and RANK. However, the capacity of conventional methods using anti-RANK antibodies to detect RANKWe sorted sub-populations of mouse bone marrow (BM) or peripheral blood (PB) cells using GST-RANKL-biotin, anti-CSF1R, and anti-B220 antibodies and induced osteoclastogenesis in vitro.The frequency of the RANKThese results suggest that GST-RANKL-biotin is useful for the detection of RANK
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::a600b041f689541d89ff4c4b11ee859d https://pubmed.ncbi.nlm.nih.gov/33516894 Zobrazit plný text záznamu Full Text from ScienceDirect