| Autor: |
P, Uher, R, Hüttelová, M, Králícková, Z, Novotný, Z, Rokyta, P, Vanderzwalmen, N, Zech |
| Jazyk: |
čeština |
| Rok vydání: |
2007 |
| Předmět: |
|
| Zdroj: |
Ceska gynekologie. 72(4) |
| ISSN: |
1210-7832 |
| Popis: |
Aim of this study was to de-differentiate the haematopoietic stem cells (HSCs) that originated from the umbilical cord blood. One of the ways to do it is to use a co-cultivation system.Prospective experimental study.Laboratory study - Institute of reproductive medicine and endocrinology, Pilsen.HSCs were co-cultivated with mouse embryonic stem cells (mESC) with and without feeder cells. After co-cultivation HSCs were analyzed using flow-cytometry for presence of haematopoietic markers (CD34, CD45, CD133) and using immunohistochemistry for presence of embryonic stem cell markers (SSEA-4, Tra-1-60, Tra-1-81).No de-differentiation was detectable in any our experiment, only the intensity of the HSC cell markers decreased.We suppose that there were two major reasons for the experiment failure: there was no direct cell to cell contact and there was a mixture of cell types that originated from two different species. To reach our goal of in vitro de-differentiation we will need to change our strategy towards a pure human culture system without any animal additives and with cell to cell contact. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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