| Autor: |
Noha M, Badawi, Yasmeen M, Attia, Dina M, El-Kersh, Olfat A, Hammam, Maha K A, Khalifa |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
International journal of nanomedicine. 17 |
| ISSN: |
1178-2013 |
| Popis: |
To design and optimizeThe quality by design approach was used to correlate the formulation parameters (PLGA amount and Poloxamer188 concentration) and critical quality attributes (entrapment efficiency percent, particle size and zeta potential). Design of CIN-PLGA-NPs formulations was done based on central composite response surface design and formulated by nanoprecipitation method. In addition, the optimized CIN-PLGA-NPs formulation was further evaluated for morphology using transmission electron microscopy and in vitro dissolution test. The cytotoxicity of CIN-PLGA-NPs optimized formula in comparison to the freeThe optimized formulation showed entrapment efficiency of 76.98%, particle size of 186.3 nm with a smooth spherical surface and zeta potential of -28.47 mV indicating its stability. Furthermore, CIN-PLGA-NPs optimized formula released 60.8±1.89% of the total CIN-Free within 24 hours compared to 29±1.25% of the raw CIN-Free indicating improved dissolution rate. The optimized formula showed superior cytotoxicity on MDA-MB-231 cells compared to its free counterpart as well as increased wound closure percentage along with reduced tumor size in mice and increased necrotic and apoptotic indices. Tumor levels of E-cadherin and N-cadherin were indicative of EMT inhibition.Our findings proved the capability of PLGA nanoparticles in loading |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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