| Autor: |
Doris, Jäger, Ursula, Müller-Werdan, Klaus, Pönicke, Jürgen, Holtz, Karl, Werdan, Sylvana P, Müller |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Molecular and cellular biochemistry. 229(1-2) |
| ISSN: |
0300-8177 |
| Popis: |
Because of its key role in proteosynthesis, the total content of elongation factor-2 (EF-2) and the distribution of six main EF-2 variants were investigated after Pseudomonas Exotoxin A catalyzed [37P]ADP-ribosylation using 1D-PAGE and isoelectric focusing (IEF) in a rat model of hemodynamic overload with variable degrees of cardiac hypertrophy: Chronic NO-synthase inhibition by L-NAME (N-omega-nitro-L-arginine-methyl-ester; 0.75 mg/ml drinking water) induced arterial hypertension without hypertrophy but myocardial apoptosis; additional treatment with IGF-1 (osmotic micropumps) did not modify hypertension but reduced apoptosis allowing moderate hypertrophy of the left ventricles. Total EF-2 did not significantly increase in rats with hemodynamic overload with or without IGF-1 supplementation. A positive correlation was found between an acidic EF-2 variant and apoptosis (p = 0.01). Hypertrophy under additional IGF-1 was combined with a shift of the EF-2 variants to basic subtypes (p0.01). This finding may be indicative of the trophic potency of IGF-1. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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