[Cultivation of continuous cell lines on the substrate of carbon nanotubes and the effect of electric stimulation on cell proliferation]

Autor: R Ia, Podcherniaeva, I A, Suetina, G R, Mikhaĭlova, O A, Lopatina, I I, Bobrinetskiĭ, R A, Morozov, A S, Seleznev
Rok vydání: 2012
Předmět:
Zdroj: Voprosy virusologii. 57(5)
ISSN: 0507-4088
Popis: It was demonstrated that the three studied samples of carbon nanotubes of domestic production fixed on the substrate surface did not have toxic effect and could be used for cell cultivation. A biocompatible conductive coating based on carbon nanotubes and bovine serum albumin was developed. The efficacy of the coating for growing in vitro cell cultures was tested. A device was developed for electric stimulation of the cells. Local electric potential was applied to the cells using nanoscale electrodes. The results of human embryonic fibroblast cultivation in a pulsed electric field on conductive nanocomposite substrates were presented. An 26% increase in the proliferative activity of cells was observed at potentials up to 100 mV.
Databáze: OpenAIRE