Amino Acid Profiling from Fingerprints Using Amide Stationary-Phase UPLC-MS

Autor: Ward, van Helmond, Marcel, de Puit
Rok vydání: 2019
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 2030
ISSN: 1940-6029
Popis: UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.
Databáze: OpenAIRE