[The enhancement of the diagnostic reliability of immunoblot for HIV-1 antibodies by enriching the preparation for immunoblot with the HIV-1 gp120 protein]

Autor:	A Iu, Zlobin, O P, Glushchenko, V M, Stakhanova, L V, Uryvaev
Rok vydání:	1992
Předmět:	Carrier State HIV Seropositivity Immunoblotting Humans Reproducibility of Results HIV Infections HIV Antibodies HIV Envelope Protein gp120 Hydrogen-Ion Concentration Immunosorbent Techniques
Zdroj:	Voprosy virusologii. 37(2)
ISSN:	0507-4088
Popis:	The diagnostic value of original immunoblot system depends on the availability of enveloped protein GP120 because it is the antibodies to this polypeptide that frequently indicate the running virus infection. This polypeptide is lost during purification of viral material but remains free in culture medium. The extraction of GP120 from culture fluid with immunosorbent based on sepharose 4B with ligated immunoglobulins from HIV-1-infected persons enriched the preparation for immunoblot with proteins increasing its diagnostic value.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::a51994055b1d14b35552b99540b888e5 https://pubmed.ncbi.nlm.nih.gov/1441433 Zobrazit plný text záznamu