MiR-590-5p regulates cell proliferation, apoptosis, migration and invasion in oral squamous cell carcinoma by targeting RECK
| Autor: | Wei-Wei, Bao, You-Ling, Shi, Yan, Ma, Xing-Hui, Qu, Guang-Ming, Pang, Lei, Yang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Squamous Cell Carcinoma of Head and Neck Apoptosis Middle Aged GPI-Linked Proteins Gene Expression Regulation Neoplastic MicroRNAs Matrix Metalloproteinase 9 Proto-Oncogene Proteins c-bcl-2 Cell Movement Case-Control Studies Cell Line Tumor Disease Progression Humans Female Mouth Neoplasms Neoplasm Invasiveness Aged Cell Proliferation Signal Transduction bcl-2-Associated X Protein |
| Zdroj: | Histology and histopathology. 36(3) |
| ISSN: | 1699-5848 |
| Popis: | To discover the role of miR-590-5p in oral squamous cell carcinoma (OSCC) progression and the corresponding mechanism via the targeting RECK.OSCC (n=85) and normal oral tissues (n=60) were collected to quantify the miR-590-5p expression by using qRT-PCR. Then SCC-15 and OEC-M1 cells were selected and divided into Mock, inhibitor NC, miR-590-5p inhibitor, si-RECK and miR-590-5p inhibitor + si-RECK groups. Dual-luciferase reporter gene assay was used to verify if miR-590-5p could target RECK. The biological behaviors of OSCC cells were evaluated by MTT, Wound-healing, Transwell and Flow cytometry. The expression of miR-590-5p and RECK was measured by qRT-PCR and Western blotting , respectively.Overexpression of miR-590-5p was found in OSCC tissues. The expression of miR-590-5p was significantly associated with the clinical TNM stage, differentiation degree, and lymph node metastasis of OSCC. RECK was identified as a direct target of miR-590-5p. Compared with the Mock group, cells in the miR-590-5p inhibitor group were decreased in terms of proliferation, invasion, and migration, and increased in cell apoptosis, accompanied by down-regulated miR-590-5p, Bcl-2/Bax and MMP-9, and up-regulated RECK. By contrast, si-RECK group presented completely opposite changes, and si-RECK reversed the inhibitory effect of miR-590-5p inhibitor on the OSCC cell growth.MiR-590-5p expression was obviously increased in OSCC, and inhibiting miR-590-5p enhanced the expression of its target gene RECK, thereby suppressing proliferation, migration and invasion of OSCC cells and promoting apoptosis of OSCC cells. |
| Databáze: | OpenAIRE |
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