[Effects of Tiam1 on invasion and metastasis of breast carcinoma and its mechanisms]
| Autor: | Yuan-qing, Wu, Yue-ying, Xie, Gang, Peng |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Mitogen-Activated Protein Kinase 1
Vascular Endothelial Growth Factor A Mitogen-Activated Protein Kinase 3 Breast Neoplasms Gene Expression Regulation Neoplastic Transcription Factor AP-1 Cell Line Tumor Lymphatic Metastasis Guanine Nucleotide Exchange Factors Humans Female Neoplasm Invasiveness T-Lymphoma Invasion and Metastasis-inducing Protein 1 Gene Silencing RNA Messenger RNA Small Interfering Signal Transduction |
| Zdroj: | Zhonghua zhong liu za zhi [Chinese journal of oncology]. 34(11) |
| ISSN: | 0253-3766 |
| Popis: | To investigate the significance of Tiam1 in invasion and metastasis of breast carcinoma and its mechanisms.Immunohistochemistry was used to detect Tiam1 expression in tumor tissue of 126 breast carcinomas. Tiam1 was silenced by siRNA in breast carcinoma cell line MDA-MB-435, then the expressions of phosphor-ERK 1, ERK 2 and VEGF were detected, and electrophoretic mobility shift assay (EMSA) was used to examine the transcription activiy of AP-1.There was a significant relationship between Tiam1 expression and lymph node metastasis (P0.05). Furthermore, after silencing of Tiam1, the expressions of phosphor-ERK 1, ERK 2 and VEGF were decreased, and the transcription activity of AP-1 was down-regulated in the MDA-MB-435 cells.Tiam1 is closely related with invasion and metastasis of breast carcinoma, and the cascade Tiam1 through ERK, AP-1 and VEGF pathways may play an important role in enhancing angiogenesis, therefore, to promote invasion and metastasis of breast carcinoma. |
| Databáze: | OpenAIRE |
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