| Autor: |
Bing-cheng, Huang, Chao, Xu, Jin, Li, Ting, Xiao, Kun, Yin, Gong-zhen, Liu, Wei-yan, Wang, Gui-hua, Zhao, Yan-bin, Wei, Yong-bin, Wang, Chang-lei, Zhao, Qing-kuan, Wei |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitologyparasitic diseases. 33(1) |
| ISSN: |
1000-7423 |
| Popis: |
To identity Plasmodium ovale infection by 18S rRNA gene nested PCR.Whole blood and filter paper blood samples of malaria patients in Shandong Province were collected during 2012-2013. The parasites were observed under a microscope with Giemsa staining. The genome DNA of blood samples were extracted as PCR templates. Genus- and species-specific primers were designed according to the Plasmodium 18S rRNA gene sequences. Plasmodium ovale-positive specimens were identified by nested PCR as well as verified by sequencing.There were 7 imported cases of P. ovale infection in the province during 2012-2013. Nested PCR results showed that the P. ovale specific band (800 bp) was amplified in all the 7 specimens. Blast results indicated that the PCR products were consistent with the Plasmodium ovale reference sequence in GenBank.Seven imported cases of ovale malaria in Shandong Province in 2012-2013 are confirmed by nested PCR. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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