| Autor: |
Ryuta, Suzuki, Noriaki, Kamio, Kozue, Sugimoto, Shuichiro, Maruoka, Yasuhiro, Gon, Tadayoshi, Kaneko, Yoshiyuki, Yonehara, Kenichi, Imai |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
In Vivo |
| ISSN: |
1791-7549 |
| Popis: |
Background/Aim: Despite evidence of an association between pulmonary diseases and periodontopathic bacteria, the molecular mechanisms remain unknown. Matrix metalloproteinase-9 (MMP9) plays important roles in pneumonia, chronic obstructive pulmonary disease, and asthma; therefore, we assessed the effects of Fusobacterium nucleatum on MMP9 expression in mouse lung and A549 human alveolar epithelial cells. Materials and Methods: Heat-killed F. nucleatum was administered to the trachea of mice or added to A549 cell cultures. MMP9 expression was determined using real-time PCR and western blotting. The involvement of mitogen-activated protein kinases (MAPKs) and nuclear factor-ĸB (NF-ĸB) in MMP9 expression was examined. Results: F. nucleatum induced expression of MMP9 in mouse lung and bronchoalveolar lavage fluid. In A549 cells, F. nucleatum induced production of MMP9 protein and mRNA in a density-dependent manner; this was inhibited by inhibitors of extracellular-regulated kinase 1/2 and NF-ĸB, but not of p38 and Jun N-terminal protein kinase. Conclusion: F. nucleatum may contribute to the onset of pulmonary diseases via MMP9 expression through extracellular-regulated kinase 1/2 and NF-ĸB activation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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