| Autor: |
G, Rocha, A, Duclos, L E, Chalifour, M G, Baines, E, Antecka, J, Deschênes |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
Canadian journal of ophthalmology. Journal canadien d'ophtalmologie. 31(5) |
| ISSN: |
0008-4182 |
| Popis: |
To analyse the pattern of gene expression at the messenger RNA level during ocular inflammation in the rabbit.A gene screen assay was used to quantify specific binding over background (expression index) of various activation markers and cytokines in rabbit iris-ciliary body obtained during active experimental uveitis induced by injection of porcine lens protein (two animals) and in a control group (two animals).Expression indices corresponding to the activation markers and cytokines assayed.Compared with the control eyes, analysis of triplicate samples from the inflamed eyes showed a significantly higher expression index corresponding to the proto-oncogenes c-fos, c-jun and Ha-ras, interleukin-2 and heat shock protein Hsp27 and a significantly lower index corresponding to transforming growth factor-beta (TGF-beta) (p0.05).Active experimental lens-induced uveitis is associated with a significant rise in the gene expression of cellular activation factors and a decrease in an immunoprotective factor (TGF-beta) in the iris and ciliary body of the rabbit. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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