| Popis: |
The ability of growth factors (GF) to stimulate proliferation of rat embryo fibroblast (REF) lines immortalized by E 1A oncogene and transformed by complementing E 1A and c-Ha-ras oncogenes (mutant form) was studied. Unlike untreated REF, those immortalized by E 1A oncogene required less serum and GF to proliferate. Proliferation could be stimulated by specific GF alone. Serum appeared to be capable of stimulating proliferation of cells transformed by E 1A + c-Ha-ras oncogenes; however, both GF and proteinases C and A activators failed to exert such effect. Transferrin, usually required by normal cells in late G1-phase, shared the stimulating effect on E 1A+c-Ha-ras-transformed cells. To summarize, E 1A+c-Ha-ras oncogene-transformed REF can grow independently of GF but still require transferrin. |
