| Autor: |
Dominic, Devost, Nicolas, Audet, Cynthia, Zhou, Hiroyuki, Kobayashi, Hélène, Bonin, Viktorya, Lukashova, Christian, Le Gouill, Michel, Bouvier, Terence E, Hébert |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Methods in cell biology. 132 |
| ISSN: |
0091-679X |
| Popis: |
The use of biosensors either individually or as part of panels has now become a common technique to capturing signaling events in living cells. Such biosensors have become particularly important for probing biased signaling and allostery in G protein-coupled receptor drug screening efforts. However, assumptions about the portability of such biosensors between cell types may lead to misinterpretation of drug effects on specific signaling pathways in a given cellular context. Further, the output of a particular biosensor may be different depending on where it is localized in a cell. Here, we discuss strategies to mitigate these concerns which should feed into future biosensor design and usage. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
