| Autor: |
Guang-Xun, Gao, Hong-Juan, Dong, Hong-Tao, Gu, Ying, Gao, Yao-Zhu, Pan, Yi-Wei, Wang, Yang, Yang, Xie-Qun, Chen |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi. 31(3) |
| ISSN: |
0253-2727 |
| Popis: |
To investigate the expression of mDia1 (mammalian diaphanous 1)in platelet and the role of mDia1 or phosphatidylinositol 3-kinase (PI3K) in the process of thrombin-induced platelet aggregation.The extent of platelet aggregation was measured by a platelet aggregation system and the expression of mDia1 and its relation with F-actin in quiescent, spreading or aggregated platelets by Western blot.There was no significant difference in mDia1 expression level between quiescent and activated platelets. mDia1 moved from a Triton-X100-soluble cytosolic fraction to insoluble cytoskeleton fraction after thrombin induced platelets aggregation. Anti-mDia1 antibody could inhibit this aggregation. PI3K inhibitor Wortmannin or Ly294002 inhibited the thrombin induced platelet aggregation and the above mentioned mDia1 translocation.PI3-kinase mediates the thrombin-induced platelet aggregation through mDia1 pathway. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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