| Autor: |
Stefan, Wilhelm, Thomas, Hirsch, Wendy M, Patterson, Elisabeth, Scheucher, Torsten, Mayr, Otto S, Wolfbeis |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Theranostics |
| ISSN: |
1838-7640 |
| Popis: |
We describe the preparation of monodisperse, lanthanide-doped hexagonal-phase NaYF4 upconverting luminescent nanoparticles for protein conjugation. Their core was coated with a silica shell which then was modified with a poly(ethylene glycol) spacer and N-hydroxysuccinimide ester groups. The nanoparticles were characterized by transmission electron microscopy, Raman spectroscopy, X-ray diffraction, and dynamic light scattering. The N-hydroxysuccinimide ester functionalization renders them highly reactive towards amine nucleophiles (e.g., proteins). We show that such particles can be conjugated to proteins. The protein-reactive UCLNPs and their conjugates to streptavidin and bovine serum albumin display multicolor emissions upon 980-nm continuous wave laser excitation. Surface plasmon resonance studies were carried out to prove bioconjugation and to compare the affinity of the particles for proteins immobilized on a thin gold film. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
