| Autor: |
Stefan, Zwingenberger, Robert, Langanke, Corina, Vater, Geoffrey, Lee, Eik, Niederlohmann, Markus, Sensenschmidt, Angela, Jacobi, Ricardo, Bernhardt, Michael, Muders, Stefan, Rammelt, Sven, Knaack, Michael, Gelinsky, Klaus-Peter, Günther, Stuart B, Goodman, Maik, Stiehler |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Journal of biomedical materials research. Part A. 104(9) |
| ISSN: |
1552-4965 |
| Popis: |
The treatment of critical size bone defects represents a challenge. The growth factor bone morphogenetic protein 2 (BMP-2) is clinically established but has potentially adverse effects when used at high doses. The aim of this study was to evaluate if stromal derived factor-1 alpha (SDF-1α) and BMP-2 released from heparinized mineralized collagen type I matrix (MCM) scaffolds have a cumulative effect on bone regeneration. MCM scaffolds were functionalized with heparin, loaded with BMP-2 and/or SDF-1α and implanted into a murine critical size femoral bone defect (control group, low dose BMP-2 group, low dose BMP-2 + SDF-1α group, and high dose BMP-2 group). After 6 weeks, both the low dose BMP-2 + SDF-1α group (5.8 ± 0.6 mm³, p = 0.0479) and the high dose BMP-2 group (6.5 ± 0.7 mm³, p = 0.008) had a significantly increased regenerated bone volume compared to the control group (4.2 ± 0.5 mm³). There was a higher healing score in the low dose BMP-2 + SDF-1α group (median grade 8; Q1-Q3 7-9; p = 0.0357) than in the low dose BMP-2 group (7; Q1-Q3 5-9) histologically. This study showed that release of BMP-2 and SDF-1α from heparinized MCM scaffolds allows for the reduction of the applied BMP-2 concentration since SDF-1α seems to enhance the osteoinductive potential of BMP-2. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2126-2134, 2016. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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