| Autor: |
Jorge, Parra-Ruiz, Marta, Alvarez, Natalia, Chueca, Alejandro, Peña, Juan, Pasquau, Miguel Angel, López-Ruz, María Del Carmen, Maroto, José, Hernández-Quero, Federico, García |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
Enfermedades infecciosas y microbiologia clinica. 27(2) |
| ISSN: |
0213-005X |
| Popis: |
Highly active antiretroviral therapy (HAART) in HIV patients is considered successful when plasma viral load (VL) reaches50 copies/ml. However, many patients have a persistent VL of 50 to 1000 copies/ml, and treatment guidelines do not recommend genotypic resistance testing at these levels because of poor performance. The aim of this study was to evaluate the usefulness of a concentration technique for HIV-1 sequencing in samples with1000 copies/ml, and determine the virological consequences of HAART treatment changes guided by resistance testing in this scenario.Observational study performed in 51 patients with plasma VL between 50 and 1000 copies/m; 27 patients had these levels for at least 12 consecutive months. Prior to RNA extraction, virions were concentrated from 3-ml plasma samples and then genotyped following standard procedures.Forty-seven of the 51 samples were successfully sequenced, resulting in a sensitivity of 92%. Among these 47 patients, 27 showed a persistent viral load of 50-1000 copies/ml for 12 months, and 20 patients achieved undetectable viral load following the genotype-guided HAART change (intention-to-treat analysis: NC = F; 20 of 27 [74.1%]; on-treatment analysis: 20 of 23 [86.9%]).We report a simple method for genotype sequencing in patients with persistent low-level viremia that allowed a modification of the HAART regimen leading to undetectable plasma viremia. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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