Autor: |
J N, Skepper, I, Karydis, M R, Garnett, L, Hegyi, S J, Hardwick, A, Warley, M J, Mitchinson, N R, Cary |
Rok vydání: |
1999 |
Předmět: |
|
Zdroj: |
The Journal of pathology. 188(1) |
ISSN: |
0022-3417 |
Popis: |
This study examines ion homeostasis in monocyte-macrophages committed to death by apoptosis. X-ray microanalysis has been used to demonstrate that intracellular concentrations of potassium decreased whilst those of sodium increased following 3 h of exposure to 100 microg/ml of oxidized low-density lipoprotein (LDL) in vitro. In contrast, the maximal incidence of cell death, as determined by the inability to exclude trypan blue, was not seen until 24 h of exposure. At 12 h, less than 1 per cent of cells were stained using terminal transferase-mediated DNA nick-end labelling, which is generally accepted as a marker of late stages in the apoptotic pathway. This is the first demonstration of early perturbations of ion homeostasis in monocyte-macrophages exposed to concentrations of oxidized LDL known to cause apoptosis. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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