Changes in elemental concentrations are associated with early stages of apoptosis in human monocyte-macrophages exposed to oxidized low-density lipoprotein: an X-ray microanalytical study

Autor:	J N, Skepper, I, Karydis, M R, Garnett, L, Hegyi, S J, Hardwick, A, Warley, M J, Mitchinson, N R, Cary
Rok vydání:	1999
Předmět:	Microscopy Electron Scanning Transmission Time Factors Iron Macrophages Sodium Apoptosis Phosphorus Lipoproteins LDL Chlorides In Situ Nick-End Labeling Potassium Homeostasis Humans Magnesium Electron Probe Microanalysis
Zdroj:	The Journal of pathology. 188(1)
ISSN:	0022-3417
Popis:	This study examines ion homeostasis in monocyte-macrophages committed to death by apoptosis. X-ray microanalysis has been used to demonstrate that intracellular concentrations of potassium decreased whilst those of sodium increased following 3 h of exposure to 100 microg/ml of oxidized low-density lipoprotein (LDL) in vitro. In contrast, the maximal incidence of cell death, as determined by the inability to exclude trypan blue, was not seen until 24 h of exposure. At 12 h, less than 1 per cent of cells were stained using terminal transferase-mediated DNA nick-end labelling, which is generally accepted as a marker of late stages in the apoptotic pathway. This is the first demonstration of early perturbations of ion homeostasis in monocyte-macrophages exposed to concentrations of oxidized LDL known to cause apoptosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::a237a0950f6093cf75c33887fc918398 https://pubmed.ncbi.nlm.nih.gov/10398148 Zobrazit plný text záznamu Plný text