| Autor: |
M T, Davis, C S, Spahr, M D, McGinley, J H, Robinson, E J, Bures, J, Beierle, J, Mort, W, Yu, R, Luethy, S D, Patterson |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Proteomics. 1(1) |
| ISSN: |
1615-9853 |
| Popis: |
With an emphasis on obtaining a multitude of high quality tandem mass spectrometry spectra for protein identification, instrumental parameters are described for the liquid chromatography-tandem mass spectrometry analysis of trypsin digested unfractionated urine using a hybrid quadrupole-time-of-flight (Q-TOF) mass spectrometer. Precursor acquisition rates of up to 20 distinct precursors/minute in a single analysis were obtained through the use of parallel precursor selection (four precursors/survey period) and variable collision induced dissociation integration time (1 to 6 periods summed). Maximal exploitation of the gas phase fractionated ions was obtained through the use of narrow survey scans and iterative data-dependent analyses incorporating dynamic exclusion. The impact on data fidelity as a product of data-dependent selection of precursor ions from a dynamically excluded field is discussed with regards to sample complexity, precursor selection rates, survey scan range and facile chemical modifications. Operational and post-analysis strategies are presented to restore data confidence and reconcile the greatest number of matched spectra. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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