Elevated plasma phage load as a marker for intestinal permeability in leukemic patients
| Autor: | Xue-Rui, Yin, Ping, Liu, Xi, Xu, Ying, Xia, Kai-Zhao, Huang, Qiong-Dan, Wang, Mei-Mei, Lai, Qi-Gui, Yu, Xiao-Qun, Zheng |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Lipopolysaccharide Receptors Antigens Differentiation Myelomonocytic Antineoplastic Agents Receptors Cell Surface Macrophage Activation Middle Aged Precursor Cell Lymphoblastic Leukemia-Lymphoma Permeability Gastrointestinal Microbiome Leukemia Myeloid Acute RNA Bacterial C-Reactive Protein Antigens CD Bacterial Translocation RNA Ribosomal 16S Humans Bacteriophages Female Viremia Intestinal Mucosa Aged |
| Zdroj: | Medical microbiology and immunology. 209(6) |
| ISSN: | 1432-1831 |
| Popis: | Microbial translocation (MT) and altered gut microbiota have been described in acute leukemic patients and contribute to immune activation and inflammation. However, phage translocation has not been investigated in leukemia patients yet. We recruited 44 leukemic patients and 52 healthy adults and quantified the levels of 3 phages in peripheral blood, which were the most positive phages screened from fecal samples. The content of 16S rRNA in plasma was detected by qPCR to assess the intestinal mucosa of these patients. Spearman's rank correlation was used to analyze the relationship between phage load and the relevant clinical data. We found the most prevalent phages in fecal samples were λ phage, Wphi phage, and P22 phage, and λ phage had the highest detection rate in plasma (68%). Phage content was affected by chemotherapy and course of disease and correlated with the levels of CRP (r = 0.43, p = 0.003), sCD14 (r = 0.37, p = 0.014), and sCD163 (r = 0.44, p = 0.003). Our data indicate that plasma phage load is a promising marker for gut barrier damage and that gut phage translocation correlates with monocyte/macrophage activation and systemic inflammatory response in leukemic patients. |
| Databáze: | OpenAIRE |
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