[Molecular and epidemiological characteristics of tick-borne encephalitis virus in the Sverdlovsk Region on the basis of genotype-specific RT-PCR]

Autor:	S Iu, Kovalev, T V, Umpeleva, T E, Snitkovskaia, A S, Kiliatsina, V V, Romanenko, V S, Kokorev, N P, Glinskikh
Rok vydání:	2008
Předmět:	Viral Proteins Ixodes Reverse Transcriptase Polymerase Chain Reaction Epidemiological Monitoring Animals Humans Arachnid Vectors Bites and Stings Sensitivity and Specificity Encephalitis Tick-Borne Encephalitis Viruses Tick-Borne Environmental Monitoring Russia
Zdroj:	Voprosy virusologii. 53(2)
ISSN:	0507-4088 2005-2006
Popis:	Studies and complete awareness of the regional and epidemiological properties of tick-borne encephalitis virus (TBEV) allow one to improve methods for preventing, diagnosing, and treating its severe neurological infection. The authors have developed reverse-transcriptase polymerase chain reaction (RT-PCR) systems for the detection of RNA of TBEV and for the determination of its genotype in the ticks and clinical materials. RT-PTC was shown to have a higher sensitivity and specificity than the practically used enzyme immunoassay system. Despite significant variations in the spread of infected ticks in some districts of the Sverdlovsk Region (5-12%), the average regional value was 8% over the study period. The authors have studied more than a thousand of ticks collected from the nature and humans in the epidemic season of 2005-2006. There was a virtually complete predominance (more than 95%) of the Ural-Siberian genotype, with rare cases of the European genotype (slightly more than 4%) being detected. The Far-Eastern genotype was not detected.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::a11734d8ce8201464c5989d0dfc6b46f https://pubmed.ncbi.nlm.nih.gov/18450106 Zobrazit plný text záznamu