N-Glycosylation analysis of yeast Carboxypeptidase Y reveals the ultimate removal of phosphate from glycans at Asn
Autor: | Gnanesh Kumar, B S, Avadhesha, Surolia |
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Rok vydání: | 2016 |
Předmět: | |
Zdroj: | International journal of biological macromolecules. 98 |
ISSN: | 1879-0003 |
Popis: | Carboxypeptidase Y from Saccharomyces cerivisiae was characterized for its site specific N-glycosylation through mass spectrometry. The N-glycopeptides were derived using non specific proteases and are analysed directly on liquid chromatography coupled to ion trap mass spectrometer in tandem mode. The evaluation of glycan fragment ions and the Y |
Databáze: | OpenAIRE |
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