N-Glycosylation analysis of yeast Carboxypeptidase Y reveals the ultimate removal of phosphate from glycans at Asn

Autor: Gnanesh Kumar, B S, Avadhesha, Surolia
Rok vydání: 2016
Předmět:
Zdroj: International journal of biological macromolecules. 98
ISSN: 1879-0003
Popis: Carboxypeptidase Y from Saccharomyces cerivisiae was characterized for its site specific N-glycosylation through mass spectrometry. The N-glycopeptides were derived using non specific proteases and are analysed directly on liquid chromatography coupled to ion trap mass spectrometer in tandem mode. The evaluation of glycan fragment ions and the Y
Databáze: OpenAIRE