| Autor: |
Li, Ling, Zheng-mao, Ye, Su-yuan, Wang, Yan, Jin, Yu-hang, Chen, Tian-li, Zheng, Li-shi, Zhang, Xiao-fang, Pei |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition. 46(5) |
| ISSN: |
1672-173X |
| Popis: |
To isolate aflatoxin-producing strains from paprika samples and to do a preliminarily study on the relationship between aflatoxin-producing ability and the genes aflR, omt-1 and ver-1.Fungi were isolated by traditional culture method. Potential aflatoxin-producing strains were screened by phenotypic traits and multiplex PCR. After these potential aflatoxin-producing strains cultured in the toxigenic culture medium, the levels of aflatoxin B, (AFB1) of the cultures were tested with ELISA method. The phylogenetic tree of aflR, omt-1 and ver-1 was constructed to explore the relationship between these genes and the AFB1-producing capacity.17 potential aflatoxin-producing fungi were isolated. The ratio of positive toxigenic strains is 64. 71%. 11 isolates were positive in AFB1 detection while existing high sequence homology with AS 3. 4408, 6 isolates were negative in AFB1 detection while existing high sequence homology with Aspergillus oryzae.Aspergillus flavus are potential candidates for aflatoxin control. Not all Aspergillus flavus have AFB1-producing capacity, aflR gene had a direct relation to AFB1-producing capacity, while ver-1 and omt-1 were related to the level of AFB1 producing. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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