Host-reactive CD4+ and CD8+ T cell clones isolated from a human chimera produce IL-5, IL-2, IFN-gamma and granulocyte/macrophage-colony-stimulating factor but not IL-4
| Autor: | R, Bacchetta, R, de Waal Malefijt, H, Yssel, J, Abrams, J E, de Vries, H, Spits, M G, Roncarolo |
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| Rok vydání: | 1990 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte CD4-Positive T-Lymphocytes Lymphokines Transcription Genetic Chimera CD8 Antigens Immunologic Deficiency Syndromes Granulocyte-Macrophage Colony-Stimulating Factor Blotting Northern Clone Cells Interferon-gamma Colony-Stimulating Factors Antigens CD Tetanus Toxoid Humans Interleukin-2 Interleukin-4 RNA Messenger Interleukin-5 Growth Substances |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 144(3) |
| ISSN: | 0022-1767 |
| Popis: | In the present study, we investigated the lymphokine production patterns in a series of CD4+ and CD8+ host-reactive T cell clones isolated from PBL of a SCID patient, who was immunologically reconstituted by two allogeneic fetal liver and thymus transplantations 13 years ago. We demonstrate that these donor-derived T cell clones, specifically reacting with the MHC Ag expressed on the recipient cells, do not produce IL-4 and do not express IL-4 mRNA upon Ag or polyclonal stimulations. In contrast, CD4+ tetanus toxin-specific T cell clones isolated from the same patient and having the same HLA phenotype produced normal amounts of IL-4 upon activation. These data suggest that the failure to produce IL-4 is a specific characteristic of these host-reactive clones and is not due to a genetic defect of the transplanted cells. Furthermore, different modes of activation resulted in simultaneous production of IL-5, IL-2, IFN-gamma, granulocyte/macrophage-CSF, and transcription of the TNF-beta gene by the host-reactive clones, indicating that the lack of IL-4 production is not related to the mode of activation. The finding that some of these clones produce significant levels of IL-5 but no IL-4 indicates that the IL-4 and IL-5 genes are not always coexpressed in activated human T cells. |
| Databáze: | OpenAIRE |
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