Isolation of novel non-HLA gene fragments from the hemochromatosis region (6p21.3) by cDNA hybridization selection
| Autor: | Goei, V. L., Parimoo, S., Capossela, A., Chu, T. W., Gruen, J. R. |
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| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities DNA Complementary Base Sequence HLA-A Antigens fungi Molecular Sequence Data Restriction Mapping Nucleic Acid Hybridization hemic and immune systems chemical and pharmacologic phenomena Blotting Northern Cell Line Blotting Southern hemic and lymphatic diseases Multigene Family Humans Chromosomes Human Pair 6 Hemochromatosis Chromosomes Artificial Yeast Sequence Analysis Research Article |
| Popis: | It has previously been shown that cDNA hybridization selection can identify and recover novel genes from large cloned genomic DNA such as cosmids or YACs. In an effort to identify candidate genes for hemochromatosis, this technique was applied to a 320-kb YAC containing the HLA-A gene. A short fragment cDNA library derived from human duodenum was selected with the YAC DNA. Ten novel gene fragments were isolated, characterized, and localized on the physical map of the YAC. |
| Databáze: | OpenAIRE |
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