| Autor: |
Nan, Hu, Jia, Sun, Yuancheng, Kang, Jiansheng, Chen, Lishan, Luo, Juchang, Zhang, Songyuan, Chen, Dehong, Cai |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 33(8) |
| ISSN: |
1673-4254 |
| Popis: |
To study the signaling pathways associated with lipopolysaccharide (LPS)-induced inflammation in islet micro-endothelial cells (IMECs) and the mechanism of pravastatin intervention.IMECs exposed to LPS, SB203580, pravastatin, or SB203580+pravastatin were examined for cell apoptosis with Hoechst staining and flow cytometry and for expression levels of total-p38, photophosphorylation-p38 (p-p38) and iNOS with Western blotting.The apoptosis rate and expression levels of total-p38, p-p38, iNOS in IMECs all increased after LPS exposure. Pravastatin, SB203580, and their combination significantly attenuated LPS-induced enhancement of cell apoptosis and total-p38, p-p38, and iNOS expressions in IMECs.LPS-induced inflammatory toxicity in IMECs is associated with the activation of P38MAPK and iNOS/NO signaling pathways. Pravastatin can inhibit these pathways and suppress the apoptosis and necrosis of IMECs to relieve the cell inflammatory injuries. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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