| Autor: |
C P, Verstijnen, J W, Arends, P, Moerkerk, B, Schutte, E, van der Linden, B, Kuypers-Engelen, F T, Bosman |
| Rok vydání: |
1988 |
| Předmět: |
|
| Zdroj: |
Anticancer research. 8(6) |
| ISSN: |
0250-7005 |
| Popis: |
In a series of 61 primary colorectal carcinomas, we attempted to determine which primary tumor characteristics correlated with the possibility to continuously maintain tumor cells in vitro or in vivo, and to what extent the characteristics of a primary tumor were maintained in vitro or as xenograft. Four continuous cell lines and 10 serially transplantable tumors were obtained. Only one cell line could be maintained both in vitro and in vivo. Tumors that had metastized and tumors in the proximal colon showed a higher success for in vivo and in vitro growth. DNA analysis showed that in most xenografts the DNA index was identical to that the primary tumor. However, in some cases tumor cell subpopulations were lost or genetically variant new subpopulations were generated. In general, the degree of differentiation in the xenografts corresponded with the least differentiated areas in the primary tumor. Xenografts appeared to display comparable of antigen expression. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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