[Searching Radiation Countermeasures using the Model of Prolonged Irradiation of Mice with Low Dose Rate and Evaluation of Their Influence on Heat Shock Protein Genes Expression]
| Autor: | L M, Rozhdestvensky, V F, Mikhailov, T G, Schlyakova, J M, Shagirova, R A, Shchegoleva, N F, Raeva, N I, Lisina, L V, Shulenina, V V, Zorin, A V, Pchelka, K Y, Trubitsina |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Time Factors Gene Expression Dose-Response Relationship Radiation Radiation-Protective Agents Radiation Dosage Real-Time Polymerase Chain Reaction Mice Inbred C57BL Radiation Injuries Experimental Bone Marrow Gamma Rays Drug Discovery Mice Inbred CBA Animals Heat-Shock Proteins Whole-Body Irradiation |
| Zdroj: | Radiatsionnaia biologiia, radioecologiia. 55(4) |
| ISSN: | 0869-8031 |
| Popis: | Different radiomodificators (cytokine betaleukine, antioxidant phenoxan, antigipoksant limontar and nucleoside riboxin) were investigated on mice for evaluating their radiation protective capacity against prolonged (21 h) exposure at a dose of 12.6 Gy at a low dose rate of 10 mGy/min. Bone marrow cellularity and endogenic CFUs were used as evaluation criteria 9 days after exposure. Simultaneously, expression of the heat shock proteins of 25, 70 and 90 kDa in unexposed mice bone marrow was studied 2, 24 and 48 h after injections. Betaleukine only had a positive significant effect in both tests in the variants of 50 mcg/kg and 3 mcg/kg when administered 2 h and 22 h before exposure, correspondingly. Effects of betaleukine HSPs on expression were both stimulating and inhibiting, that was in contradiction with a constant positive effect in 5 experiments on exposed mice for each betaleukine variant. It argues against the vital role of HSPs in the betaleukine antiradiation effect. In 2 experiments with high temperatures betaleukine administered at a dose of 50 mcg/kg evoked a very high HSP-70 gene expression after 24 h, and mice exposed to irradiation at that time in a parallel experiment showed an increased radiation effect. It corresponds to the idea that HSPs serve a stress indicator. |
| Databáze: | OpenAIRE |
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