Restoration of thymopoiesis in pT alpha-/- mice by anti-CD3epsilon antibody treatment or with transgenes encoding activated Lck or tailless pT alpha
| Autor: | H J, Fehling, B M, Iritani, A, Krotkova, K A, Forbush, C, Laplace, R M, Perlmutter, H, von Boehmer |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Mice
Knockout Cytoplasm CD3 Complex Receptors Antigen T-Cell alpha-beta T-Lymphocytes Cell Differentiation Mice Transgenic Thymus Gland Mice Inbred C57BL Mice Structure-Activity Relationship src-Family Kinases Lymphocyte Specific Protein Tyrosine Kinase p56(lck) Animals Transgenes Sequence Deletion Signal Transduction |
| Zdroj: | Immunity. 6(6) |
| ISSN: | 1074-7613 |
| Popis: | Mice deficient for the pre-TCR alpha (pT alpha) chain cannot form a pre-T cell receptor (TCR) and exhibit a severe defect in early T cell development, characterized by lack of "beta selection" and impaired generation of double-positive (DP) thymocytes. Here, we demonstrate that intraperitoneal injection of CD3epsilon-specific antibodies into pT alpha-/- x RAG-/- mice or introduction of an activated p56(lck) transgene in pT alpha-/- mice fully restores the number of DP thymocytes, and that expression of a transgenic pT alpha chain lacking its cytoplasmic portion can overcome all developmental defects associated with pT alpha deficiency. These results allow a better definition of the role of pT alpha in pre-TCR signal transduction and provide conclusive evidence that the cytoplasmic tail of pT alpha is not essential for pre-TCR signaling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|