Biocompatibility of membranes with unrestricted somatic stem cells
| Autor: | Christian, Naujoks, Felix Paulssen, Von Beck, Fabian, Langenbach, Michael, Hentschel, Karin, Berr, Matthias, Hofer, Rita, Depprich, Norbert, Kübler, Jörg, Handschel |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Osteoblasts Cell Survival Stem Cells Reproducibility of Results Biocompatible Materials Cell Differentiation Membranes Artificial Ascorbic Acid Dexamethasone Osteogenesis Glycerophosphates Materials Testing Cell Adhesion Microscopy Electron Scanning Humans Female Collagen Polytetrafluoroethylene Cells Cultured Cell Proliferation |
| Zdroj: | In vivo (Athens, Greece). 27(1) |
| ISSN: | 1791-7549 |
| Popis: | The biocompatibility of human osteoblasts (HOB) and human unrestricted somatic stem cells (USSCs) with membranes (BioGide®, GORE-TEX®, GENTA-FOIL resorb®, RESODONT®, BioMend®, BioMend® Extend™) was evaluated.After osteogenic differentiation (dexamethasone, ascorbic acid and β-glycerolphosphate) cells were seeded on membranes. On days 1, 3 and 7, attachment, proliferation, cell vitality, cytotoxicty and cell morphology were analyzed.Cells on BioGide® and RESODONT® exhibited significantly higher attachment (p0.005) and proliferation (p0.005). On BioMend® cells showed a significantly higher attachment compared to BioMend® Extend™ (p0.005), whereas on BioMend® Extend™ cells had significantly higher proliferation (p0.005). The vitality of cells was significantly better on BioGide® and RESODONT® (p0.005). There were no significant differences between USSCs and HOBs. Scanning electron microscopy confirmed these results.BioGide® and RESODONT® had the best biocompatibility and are appropriate membranes for use in stem cell-derived regeneration of bone. |
| Databáze: | OpenAIRE |
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