| Autor: |
I, Scheer, M D, Ludevid, F, Regad, B, Lescure, R F, Pont-Lezica |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
Plant molecular biology. 35(6) |
| ISSN: |
0167-4412 |
| Popis: |
The promoter of a gene encoding a ribosome-associated protein of 40 kDa from Arabidopsis thaliana (A-p40) was sequenced and the expression of the gene studied. A-p40 was expressed in the same organs and with the same variations as the eukaryotic elongation factor 1 alpha (eEF1A), another gene coding for a protein involved in translation Arabidopsis plants transformed with a beta-glucuronidase (GUS) gene driven by the A-p40 promoter confirm that A-p40 is expressed in actively dividing and growing cells. eEF1A promoter-GUS fusions have the same pattern of expression. Comparison of cis-acting elements from A-p40 and eEF1A revealed some common elements. A-p40 promoter deletions and transient gene expression in transfected Arabidopsis protopasts allowed the identification of trap40, a cis-acting element regulating gene expression. Gel retardation experiments indicate that eEF1A and A-p40 are regulated by different cis-acting elements. The role of such elements is discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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