Amniotic fluid phospholipids measured by continuous-development thin-layer chromatography

Autor: M D, Kolins, E, Epstein, W H, Civin, S, Weiner
Rok vydání: 1980
Předmět:
Zdroj: Clinical chemistry. 26(3)
ISSN: 0009-9147
Popis: We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.
Databáze: OpenAIRE