Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers
| Autor: | D, Sehgal, E, Schiaffella, A O, Anderson, R G, Mage |
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| Rok vydání: | 2001 |
| Předmět: |
Gene Rearrangement
B-Lymphocytes Base Sequence Genes Immunoglobulin Molecular Sequence Data Gene Conversion Immunoglobulin Variable Region Germinal Center Complementarity Determining Regions Dinitrobenzenes Mutation Animals Immunoglobulin Light Chains Amino Acid Sequence Rabbits Immunoglobulin Heavy Chains Spleen |
| Zdroj: | European journal of immunology. 30(12) |
| ISSN: | 0014-2980 |
| Popis: | The mechanisms described here account for development of the heterogeneous high-affinity anti-DNP antibodies that rabbits can produce. Rearranged immunoglobulin light and heavy chain genes from single DNP-specific splenic germinal center B cells were amplified by PCR. We found that in clonal lineages, rearranged V[kappa] and V[H] are further diversified by gene conversion and somatic hypermutation. The positive and negative selection of amino acids in complementarity-determining regions observed allows emergence of a variety of different combining site structures. A by-product of the germinal center reaction may be cells with sequences altered by gene conversion that no longer react with the immunizing antigen but are a source of new repertoire. The splenic germinal center would thus play an additional role in adults similar to that of the appendix and other gut-associated lymphoid tissues of young rabbits. |
| Databáze: | OpenAIRE |
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