Differential adrenergic regulation of the circadian expression of the clock genes Period1 and Period2 in the rat pineal gland

Autor:	S, Takekida, L, Yan, E S, Maywood, M H, Hastings, H, Okamura
Rok vydání:	2000
Předmět:	Male Time Factors Light Transcription Genetic Arylamine N-Acetyltransferase Nuclear Proteins Cell Cycle Proteins Period Circadian Proteins Superior Cervical Ganglion Darkness Pineal Gland Ganglionectomy Circadian Rhythm Rats Gene Expression Regulation Biological Clocks Animals RNA Messenger Rats Wistar Transcription Factors
Zdroj:	The European journal of neuroscience. 12(12)
ISSN:	0953-816X
Popis:	Precise temporal regulation of transcription is pivotal to the role of the mammalian pineal gland as a transducer of circadian and seasonal information. The circadian clock genes Per1 and Per2 encode factors implicated in temporally gated transcriptional programmes in brain and pituitary. Here we show that the nocturnal circadian expression of Per1 and Per2 in the rat pineal gland parallels that of serotonin N-acetyltransferase (NAT) mRNA, which encodes the rate-limiting enzyme of melatonin biosynthesis. This rhythm is dependent upon an intact sympathetic innervation. Increases in rPer1 (r indicates rat) and rPer2, as well as rNAT, expression during subjective night were blocked completely by superior cervical ganglionectomy (SCGX). In SCGX rats, the beta-adrenergic receptor agonist isoproterenol rapidly induced the rPer1 mRNA with dynamics very similar to its effect on rNAT mRNA. In contrast, isoproterenol was without effect on expression of rPer2 mRNA. These findings demonstrate that circadian pineal expression of both rPer1 and rPer2 is controlled by sympathetic afferent innervation, but whereas beta-adrenergic signalling regulates rPer1 and rNAT, an alternative route mediates sympathetic regulation over rPer2 expression.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::9e9ce788b913e91d61042aeaaf8bd6f5 https://pubmed.ncbi.nlm.nih.gov/11122368 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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